Warra Tall Eucalypt SuperSite – Global Malaise Program – Terrestrial Arthropod DNA Barcoding

The Global Malaise Program (GMP) is an international collaboration between the University of Guelph, Centre for Biodiversity Genomics (CBG) and international contributors (44 sites across 27 countries).

A single Malaise trap was deployed at the Warra Tall Eucalypt SuperSite in April 2014 and samples collected on a weekly basis for a year. A total of 52 samples were collected and sent to CBG in Guelph, Ontario, Canada for DNA barcoding.

At CBG, samples were accessioned and twenty-six bi-weekly samples were processed. Standard barcoding protocols (http://ccdb.ca/resources) were followed to recover the barcode region of cytochrome c oxidase subunit I (COI) gene. Barcoded specimens were assigned to an existing or new Barcode Index Number (BIN), a proxy for a formal Linnean species name. When a unique BIN was encountered (new BINs to the Barcode of Life Data Systems, BOLD (http://www.boldsystems.org), the voucher was retrieved from the specimen archive and photographed. All barcode sequences, specimen images and collateral data are stored online and the project is available in the ‘Global Malaise Program’ campaign on BOLD. Identifications were assigned by the BOLD-ID Engine where possible, allowing a preliminary species inventory to be completed for this location and facilitating comparisons among other samplings sites in GMP.

There were a total of 39 144 specimens and a barcode recovery rate of 88% was observed. The vast majority of specimens were flies (Diptera), followed in abundance by bees, ants and wasps (Hymenoptera) and mites (Acari). An excess amount of springtails (Collembola) was observed and approximately a thousand individuals were excluded from sequencing. A total of 2093 BINs were documented and 79% are new to BOLD as of September 2016. At least 50% of the BINs of each taxon are unique to this site.

Barcoding data available from Barcode of Life Data Systems (http://www.boldsystems.org).

Warra Tall Eucalypt SuperSite GMP Project update September 2016 is available here