public read uid=supersite,o=unaffiliated,dc=ecoinformatics,dc=org all uid=hero,o=unaffiliated,dc=ecoinformatics,dc=org all uid=karan,o=unaffiliated,dc=ecoinformatics,dc=org all Professor Peter Grace QUT Theme Leader, Healthy Ecosystems and Environmental Monitoring.

Institute for Future Environments. Level 7, P Block, Gardens Point Campus, GPO Box 2434 Brisbane QLD 4001 Australia

07 3138 1284 serf@qut.edu.au Soil collection and analysis of chemical and physical attributes was carried out to provide contextual data for the Biomes of Australian Soil Environments (BASE) soil microbial diversity project (http://www.bioplatforms.com/soil-biodiversity/). Nine discrete soil samples from a 25 m x 25 m quadrat, sampled at two depth ranges (0 – 10cm and 20 – 30cm). Eight samples were taken at the corners and mid-points of the 25 m x 25m sides of the quadrat, with sample taken at the centre. The nine subsamples were combined for each depth, to return a single surface and deeper soil sample per quadrat. Samples for chemical and physical analysis were air-dried and transported to CSBP laboratories, Perth, Australia. 0501 0502 0503 anzsrc-for soil physico-chemical chemistry core 1 ha SEQP SEQ Peri-urban SuperSite Samford pasture lawn Creative Commons Attribution 4.0 International License 2013 Samford Ecological Research Facility, Samford 152.88 152.88 -27.39 -27.39 1449411748736

Soil samples were collected according to the methods described at the BASE dataportal (http://www.bioplatforms.com/sample-collection-procedure). Briefly, nine discrete soil samples from a 25 m x 25 m quadrat, sampled at two depth ranges (0 – 10 cm and 20 – 30 cm). Eight samples were taken at the corners and mid-points of the 25 m x 25 m sides of the quadrat, with sample taken at the centre. The nine subsamples were combined for each depth, to return a single surface and deeper soil sample per quadrat. Samples for chemical and physical analysis were air-dried and transported to CSBP laboratories, Perth, Australia (http://www.csbp-fertilisers.com.au/csbp-lab).

Soil moisture (%GWC) was measured gravimetrically, ammonium and nitrate were determined colorometrically, following extraction with 1M potassium chloride (25˚C). Available phosphorus and potassium were measured using the Colwell method. Sulphur was determined by the Blair/Lefroy Extractable Sulphur method. Organic carbon was determined using the Walkley-Black method. For pH, CaCl pH and electrical conductivity (EC1:5), soils were extracted in deionised water for 1 hour to achieve a soil: solution ratio of 1:5. The water pH and EC1:5 of the extract were subsequently measured using a combination pH electrode. After water pH and EC were measured, calcium chloride solution was added to the soil solution and after thorough mixing the calcium chloride pH determined. Diethylene-triamine-pentaacetic acid (DTPA) trace elements (Cu, Fe, Mn, Zn) were determined by atomic absorption spectroscopy following extraction with (DPTA) for 2 hours. Soils were extracted with a 0.01 M calcium chloride solution and analysed for extractable Aluminium on Inductively Coupled Plasma Spectroscopy (ICP). Boron was measured by ICP after hot CaCl2 extraction. Soil exchangeable cations (Mg, K, Na,Ca) were determined using a 1:5 soil:water extraction. This test was used in combination with the NH4Cl2/ BaCl2 extractable exchangeable cations test, where the value for water soluble exchangeable cations is subtracted from the value for NH4Cl2/ BaCl2 extractable exchangeable cations. Soil particle size was also measured. Soil were sieved to 2 mm (> 2mm = gravel), treated with hydrogen peroxide to remove the organic matter, then treated with a 1:1 Calgon - Sodium Hydroxide mixture to disperse particles. Using a standardized table of particle sedimentation times, 25ml aliquots were removed from the shaken sample and the remaining sample sieved. The samples were evaporated, oven dried and weighed to determine the sand, silt and clay contents. Ref: Bissett et al. Introducing BASE - The Biomes of Australian Soil Environments soil microbial diversity database. GigaScience (Submitted)

asn_seqp_samford_core_1_ha_soil_physico_chem_2013.csv BASE Contextual Soil Physico Chemical Data, Samford Core 1 ha, 2013 asn_seqp_samford_core_1_ha_soil_physico_chem_2013.csv 1307 1 #x0A column , ecogrid://knb/supersite.398.1 cn=allusers,o=unaffiliated,dc=ecoinformatics,dc=org read uid=grace,o=unaffiliated,dc=ecoinformatics,dc=org all uid=supersite,o=unaffiliated,dc=ecoinformatics,dc=org all uid=karan,o=unaffiliated,dc=ecoinformatics,dc=org all BioPlatforms BASE ID Identifying code used in the BASE project ID Code Date sampled Date sampled dd/mm/yyyy 0 lat (-) in decimal degrees Latitude in decimal degrees degree natural long in decimal degrees Longitude in decimal degrees degree natural Depth Depth of sampling centimeter natural Horizon controlled vocab (1) Horizon horizon Description Description of sampling site site description Elevation (m) Altitude above sea level meter natural Slope (%) Slope of site Percentage natural Slope Aspect (Direction or degrees; e.g., NW or 315�) Direction or degrees; e.g., NW or 315 slope aspect Australian Soil Classification Australian Soil Classification Australian Soil Classification Soil moisture (%) Gravimetric soil moisture Percentage natural Gravel (%) - ( >2.0 mm) Gravel content Percentage natural Texture () Texture texture Course Sand (%) (200-2000 �m) Course Sand (200-2000 µm) content Percentage natural Fine Sand (%) - (20-200 �m) Fine Sand (20-200 µm) content Percentage natural Sand (%) Sand content Percentage natural Silt (%) (2-20 �m) Silt (2-20 µm) content Percentage natural Clay (%) (<2 �m) Clay (<2 µm) content Percentage natural Ammonium Nitrogen (mg/Kg) Ammonium Nitrogen (mg/Kg) milligramsPerKilogram natural Nitrate Nitrogen (mg/Kg) Nitrate Nitrogen (mg/Kg) milligramsPerKilogram natural Phosphorus Colwell (mg/Kg) Phosphorus Colwell (mg/Kg) milligramsPerKilogram natural Potassium Colwell (mg/Kg) Potassium Colwell (mg/Kg) milligramsPerKilogram natural Sulphur (mg/Kg) Sulphur (mg/Kg) milligramsPerKilogram natural Organic Carbon (%) Organic Carbon (%) Percentage natural Conductivity (dS/m) Conductivity (dS/m) Micro Siemens natural pH Level (CaCl2) (pH) pH Level (CaCl2) (pH) pH natural pH Level (H2O) (pH) pH Level (H2O) (pH) pH natural DTPA Copper (mg/Kg) DTPA Copper (mg/Kg) milligramsPerKilogram natural DTPA Iron (mg/Kg) DTPA Iron (mg/Kg) milligramsPerKilogram natural DTPA Manganese (mg/Kg) DTPA Manganese (mg/Kg) milligramsPerKilogram natural DTPA Zinc (mg/Kg) DTPA Zinc (mg/Kg) milligramsPerKilogram natural Exc. Aluminium (meq/100g) Exc. Aluminium (meq/100g) milliequivalentPer100grams natural Exc. Calcium (meq/100g) Exc. Calcium (meq/100g) milliequivalentPer100grams natural Exc. Magnesium (meq/100g) Exc. Magnesium (meq/100g) milliequivalentPer100grams natural Exc. Potassium (meq/100g) Exc. Potassium (meq/100g) milliequivalentPer100grams natural Exc. Sodium (meq/100g) Exc. Sodium (meq/100g) milliequivalentPer100grams natural Boron Hot CaCl2 (mg/Kg) Boron Hot CaCl2 (mg/Kg) milligramsPerKilogram natural 3 asn_seqp_samford_lawn_soil_physico_chem_2013.csv BASE Contextual Soil Physico-Chemical Data, Samford, Lawn Site, 2013 asn_seqp_samford_lawn_soil_physico_chem_2013.csv 1340 1 #x0A column , ecogrid://knb/supersite.399.1 cn=allusers,o=unaffiliated,dc=ecoinformatics,dc=org read uid=grace,o=unaffiliated,dc=ecoinformatics,dc=org all uid=supersite,o=unaffiliated,dc=ecoinformatics,dc=org all uid=karan,o=unaffiliated,dc=ecoinformatics,dc=org all BioPlatforms BASE ID Identifying code used in the BASE project ID Code Date sampled Date sampled dd/mm/yyyy 0 lat (-) in decimal degrees Latitude in decimal degrees degree natural long in decimal degrees Longitude in decimal degrees degree natural Depth Depth of sampling centimeter natural Horizon controlled vocab (1) Soil horizon horizon Description Description of sampling site Site description Elevation (m) Altitude above sea level meter natural Slope (%) Slope of site Percentage natural Slope Aspect (Direction or degrees; e.g., NW or 315�) Direction or degrees; e.g., NW or 315 slope aspect Australian Soil Classification Australian Soil Classification soil classification Soil moisture (%) Gravimetric soil moisture Percentage natural Gravel (%) - ( >2.0 mm) Gravel content Percentage natural Texture Texture texture Course Sand (%) (200-2000 �m) Course Sand (200-2000 µm) content Percentage natural Fine Sand (%) - (20-200 �m) Fine Sand (20-200 µm) content Percentage natural Sand (%) Sand content Percentage natural Silt (%) (2-20 �m) Silt (2-20 µm) content Percentage natural Clay (%) (<2 �m) Clay (<2 µm) content Percentage natural Ammonium Nitrogen (mg/Kg) Ammonium Nitrogen (mg/Kg) milligramsPerKilogram natural Nitrate Nitrogen (mg/Kg) Nitrate Nitrogen (mg/Kg) milligramsPerKilogram natural Phosphorus Colwell (mg/Kg) Phosphorus Colwell (mg/Kg) milligramsPerKilogram natural Potassium Colwell (mg/Kg) Potassium Colwell (mg/Kg) milligramsPerKilogram natural Sulphur (mg/Kg) Sulphur (mg/Kg) milligramsPerKilogram natural Organic Carbon (%) Organic Carbon (%) Percentage natural Conductivity (dS/m) Conductivity Micro Siemens natural pH Level (CaCl2) (pH) pH Level (CaCl2) (pH) pH natural pH Level (H2O) (pH) pH Level (H2O) (pH) pH natural DTPA Copper (mg/Kg) DTPA Copper (mg/Kg) milligramsPerKilogram natural DTPA Iron (mg/Kg) DTPA Iron (mg/Kg) milligramsPerKilogram natural DTPA Manganese (mg/Kg) DTPA Manganese (mg/Kg) milligramsPerKilogram natural DTPA Zinc (mg/Kg) DTPA Zinc (mg/Kg) milligramsPerKilogram natural Exc. Aluminium (meq/100g) Exc. Aluminium (meq/100g) milliequivalentPer100grams natural Exc. Calcium (meq/100g) Exc. Calcium (meq/100g) milliequivalentPer100grams natural Exc. Magnesium (meq/100g) Exc. Magnesium (meq/100g) milliequivalentPer100grams natural Exc. Potassium (meq/100g) Exc. Potassium (meq/100g) milliequivalentPer100grams natural Exc. Sodium (meq/100g) Exc. Sodium (meq/100g) milliequivalentPer100grams natural Boron Hot CaCl2 (mg/Kg) Boron Hot CaCl2 (mg/Kg) milligramsPerKilogram natural 2 asn_seqp_samford_pasture_soil_physico_chem_2013.csv BASE Contextual Soil Physico-Chemical Data, Samford, Pasture Site, 2013 asn_seqp_samford_pasture_soil_physico_chem_2013.csv 1770 1 #x0A column , ecogrid://knb/supersite.400.1 cn=allusers,o=unaffiliated,dc=ecoinformatics,dc=org read uid=grace,o=unaffiliated,dc=ecoinformatics,dc=org all uid=karan,o=unaffiliated,dc=ecoinformatics,dc=org all uid=supersite,o=unaffiliated,dc=ecoinformatics,dc=org all BioPlatforms BASE ID Identifying code used in the BASE project ID Code Date sampled Date sampled dd/mm/yyyy 0 lat (-) in decimal degrees Latitude in decimal degrees degree natural long in decimal degrees Longitude in decimal degrees degree natural Depth Depth of sampling centimeter natural Horizon controlled vocab (1) Soil horizon horizon Description Description of sampling site Site description Elevation (m) Altitude above sea level meter natural Slope (%) Slope of site Percentage natural Slope Aspect (Direction or degrees; e.g., NW or 315�) Direction or degrees; e.g., NW or 315 slope aspect Australian Soil Classification Australian Soil Classification soil classification Soil moisture (%) Gravimetric soil moisture Percentage natural Gravel (%) - ( >2.0 mm) Gravel content Percentage natural Texture () Texture texture Course Sand (%) (200-2000 �m) Course Sand (200-2000 µm) content Percentage natural Fine Sand (%) - (20-200 �m) Fine Sand (20-200 µm) content Percentage natural Sand (%) Sand content Percentage natural Silt (%) (2-20 �m) Silt (2-20 µm) content Percentage natural Clay (%) (<2 �m) Clay (<2 µm) content Percentage natural Ammonium Nitrogen (mg/Kg) Ammonium Nitrogen (mg/Kg) milligramsPerKilogram natural Nitrate Nitrogen (mg/Kg) Nitrate Nitrogen (mg/Kg) milligramsPerKilogram natural Phosphorus Colwell (mg/Kg) Phosphorus Colwell (mg/Kg) milligramsPerKilogram natural Potassium Colwell (mg/Kg) Potassium Colwell (mg/Kg) milligramsPerKilogram natural Sulphur (mg/Kg) Sulphur (mg/Kg) milligramsPerKilogram natural Organic Carbon (%) Organic Carbon (%) Percentage natural Conductivity (dS/m) Conductivity (dS/m) Micro Siemens natural pH Level (CaCl2) (pH) pH Level (CaCl2) (pH) pH natural pH Level (H2O) (pH) pH Level (H2O) (pH) pH natural DTPA Copper (mg/Kg) DTPA Copper (mg/Kg) milligramsPerKilogram natural DTPA Iron (mg/Kg) DTPA Iron (mg/Kg) milligramsPerKilogram natural DTPA Manganese (mg/Kg) DTPA Manganese (mg/Kg) milligramsPerKilogram natural DTPA Zinc (mg/Kg) DTPA Zinc (mg/Kg) milligramsPerKilogram natural Exc. Aluminium (meq/100g) Exc. Aluminium (meq/100g) milliequivalentPer100grams natural Exc. Calcium (meq/100g) Exc. Calcium (meq/100g) milliequivalentPer100grams natural Exc. Magnesium (meq/100g) Exc. Magnesium (meq/100g) milliequivalentPer100grams natural Exc. Potassium (meq/100g) Exc. Potassium (meq/100g) milliequivalentPer100grams natural Exc. Sodium (meq/100g) Exc. Sodium (meq/100g) milliequivalentPer100grams natural Boron Hot CaCl2 (mg/Kg) Boron Hot CaCl2 (mg/Kg) milligramsPerKilogram natural 4 per cent milligramsPerKilogram Micro Siemens pH milliequivalentPer100grams